So far today I have already looked at and analyzed two of the datasets from yesterday. Neither one had a single reliable interaction. It takes roughly forty minutes for matlab to perform the analysis after we select our points so I have been spending that time trying to outline the flow for a new matlab program that would make use of the quantum dots to determine the position of the protiens. This is a neccessary step to improve the accuracy of our results and i'm sure would greatly benefit Rachid if i can make it work.
In the meantime Rachid is performing some experiments with NucS, specifically testing the number of nonspecific interactions that still occur even with a surface that has been passivated. So there is no DNA on the surface only our blocking reagent and the streptavidin.
Update: have now completed seven different analyses within one day. The number of reliable interactions are much fewer than we would have liked and a lot of them have too short of a time interval to be sure they interacted at all.
No comments:
Post a Comment